To deprive REM sleep, the jar pot method is used. In this method, the animals are placed in a medium-sized cage in the middle of which there is an acrylic platform 2 cm high and 6 cm in diameter and the cage is filled with water to a height of 1 cm. In this situation, the animal can go to Non-REM sleep, but since the onset of REM sleep is accompanied by relaxation of the axial muscles of the neck, as soon as the animal enters the REM phase, the snout comes into contact with water and wakes . The animals in the control group are in the same position, with the difference that the diameter of the platforms is larger and about 12 cm, and the animals can easily fall asleep on it. REM sleep deprivation is performed for 24, 48 or 72 hours, and immediately after 72 hours, the relevant test is performed.